Sulfur-containing spiroketals from Breynia disticha and evaluations of their anti-inflammatory effect

• Ken-ichi Nakashima,
• Naohito Abe,
• Masayoshi Oyama,
• Hiroko Murata and
• Makoto Inoue

Beilstein J. Org. Chem. 2023, 19, 1604–1614, doi:10.3762/bjoc.19.117

• the LPS-only group)]; d) concentration-dependent cytotoxicity of 7 in RAW 264.7 cells (cell viability was detected by an MTT-based colorimetric assay); e) mRNA levels of IL-1β and IL-6 in RAW 264.7 cells preincubated with different concentrations of compound 7 for 24 h, followed by treatment with LPS

Synthesis and biological evaluation of Argemone mexicana-inspired antimicrobials

• Jessica Villegas,
• Bryce C. Ball,
• Katelyn M. Shouse,
• Caleb W. VanArragon,
• Ashley N. Wasserman,
• Hannah E. Bhakta,
• Allen G. Oliver,
• Danielle A. Orozco-Nunnelly and
• Jeffrey M. Pruet

Beilstein J. Org. Chem. 2023, 19, 1511–1524, doi:10.3762/bjoc.19.108

• A. mexicana extract [9]. All variants were assessed against T84 colon cancer cells, using the MTT colorimetric assay, and compared to the parent compounds berberine or chelerythrine. As seen in Figure 5, several berberine variants showed fairly dynamic effects on the cancer cell viability, while

• inhibition. Effects of original berberine and all variants against T84 human colon cancer cells. Cells were treated for 1 h with 20 μL of a 6 mg/mL solution, resulting in treatments of 0.12 mg of the compound of interest. The MTT colorimetric assay was used to determine cell metabolic activity after

• corresponding P-value displayed above. Effects of original chelerythrine and all variants against T84 human colon cancer. Cells were treated for 1 h with 5 μL of a 6 mg/mL solution, resulting in treatments of 0.03 mg of the compound of interest. The MTT colorimetric assay was used to determine cell metabolic

New azodyrecins identified by a genome mining-directed reactivity-based screening

• Atina Rizkiya Choirunnisa,
• Kuga Arima,
• Yo Abe,
• Noritaka Kagaya,
• Kei Kudo,
• Hikaru Suenaga,
• Junko Hashimoto,
• Manabu Fujie,
• Noriyuki Satoh,
• Kazuo Shin-ya,
• Kenichi Matsuda and
• Toshiyuki Wakimoto

Beilstein J. Org. Chem. 2022, 18, 1017–1025, doi:10.3762/bjoc.18.102

• in the Refseq database. Nodes are colored according to the host organism’s order. Enzymes with known biosynthetic products are colored red. N2H4-detecting colorimetric assay. Proposed biosynthetic pathway of azodyrecin. 1H (500 MHz) and 13C (125 MHz) NMR data for azodyrecin D (7), azodyrecin E (8

Vicinal difluorination as a C=C surrogate: an analog of piperine with enhanced solubility, photostability, and acetylcholinesterase inhibitory activity

• Yuvixza Lizarme-Salas,
• Alexandra Daryl Ariawan,
• Ranjala Ratnayake,
• Hendrik Luesch,
• Angela Finch and
• Luke Hunter

Beilstein J. Org. Chem. 2020, 16, 2663–2670, doi:10.3762/bjoc.16.216

• (BACE-1). The inhibition of AChE was measured using a modification of a previously described colorimetric assay (Figure 4a) [33]. It quickly became apparent that the limited solubility of piperine (1) in 50 mM Tris-HCl buffer, even in the presence of methanol as a co-solvent, was a major problem in this

Diversity-oriented synthesis of spirothiazolidinediones and their biological evaluation

• Sambasivarao Kotha,
• Gaddamedi Sreevani,
• Lilya U. Dzhemileva,
• Milyausha M. Yunusbaeva,
• Usein M. Dzhemilev and
• Vladimir A. D’yakonov

Beilstein J. Org. Chem. 2019, 15, 2774–2781, doi:10.3762/bjoc.15.269

• × 105 cells per 24 well plates in RPMI with 10% FBS. MTT Assay: The MTT assay is a colorimetric assay for evaluation of cell metabolic activity. The NADPH-dependent cellular oxidoreductases present in the living cell can reflect, under certain conditions, the cell viability. These enzymes can reduce the

• dormancy) of drug candidates and toxic compounds. In vitro cytotoxicity was assessed using a standard MTT colorimetric assay in a similar manner as described in [60][61]. HEK293 cells were plated in 96-well microassay culture plates (1 × 104 cells per well) and grown overnight at 37 °C in a 5% CO2

Revisiting ring-degenerate rearrangements of 1-substituted-4-imino-1,2,3-triazoles

• James T. Fletcher,
• Matthew D. Hanson,
• Joseph A. Christensen and
• Eric M. Villa

Beilstein J. Org. Chem. 2018, 14, 2098–2105, doi:10.3762/bjoc.14.184

• facilitated by a high throughput colorimetric assay to directly monitor the generation of a 4-nitroaniline byproduct. Keywords: colorimetric assay; condensation; imine exchange; rearrangement; 1,2,3-triazole; Introduction Examples of multidentate chelators comprised of 1,2,3-triazole units have surged in

• proposed advantages for using 1a in preparing formyltriazole products, while also examining how amine identity impacts rearrangement rates, a high-throughput colorimetric assay exploiting the electronic properties of the para-nitroaniline byproduct was developed. DMSO was selected as the reaction solvent

Glyco-gold nanoparticles: synthesis and applications

• Federica Compostella,
• Olimpia Pitirollo,
• Alessandro Silvestri and
• Laura Polito

Beilstein J. Org. Chem. 2017, 13, 1008–1021, doi:10.3762/bjoc.13.100

• , on the base of different expression level of the bacterial lectin FimH with a colorimetric assay. A simple and fast bioassay has been developed by Lee et al. [83] to recognize cholera toxin (CT), a protein secreted by the Vibrio cholerae bacterium which is responsible for cholera disease. A thiol

• )galactose chains [85]. GAuNPs functionalized with the exact sequence have been able to selectively recognize the influenza virus strain by means of a simple colorimetric assay, observing the hemagglutinin-induced aggregation of AuNPs [44][45]. Moreover, to improve the sialic residue display on metal

Synthesis of nanodiamond derivatives carrying amino functions and quantification by a modified Kaiser test

• Gerald Jarre,
• Steffen Heyer,
• Elisabeth Memmel,
• Thomas Meinhardt and
• Anke Krueger

Beilstein J. Org. Chem. 2014, 10, 2729–2737, doi:10.3762/bjoc.10.288

• solution should be executed only after the disappearance of the red colour. The back oxidation is otherwise hindered and a quantitative determination is rendered impossible (Figure 3). In order to calibrate the measurement a reference sample for the colorimetric assay was used. Using benzylamine at

• nanodiamond 5 with the same protocol and taking samples of 1 mL at time intervals. After work-up these samples were submitted to the colorimetric assay and a minimum duration until complete transformation was found to be in the range of 10 min. Using this optimized assay protocol a series of aminated

• File 414: Details for calibration of the Kaiser test, test robustness and optimization of the reaction time, reaction mechanism for colorimetric assay; synthesis of organic precursor compounds and further nanodiamond derivatives. Acknowledgements We gratefully acknowledge the financial support by the

SF002-96-1, a new drimane sesquiterpene lactone from an Aspergillus species, inhibits survivin expression

• Silke Felix,
• Louis P. Sandjo,
• Till Opatz and
• Gerhard Erkel

Beilstein J. Org. Chem. 2013, 9, 2866–2876, doi:10.3762/bjoc.9.323

• shown as mean values ± SD of three independent experiments. SF002-96-1 induces apoptosis of Colo 320 cells. (A) Colo 320 cells were treated without (control) or with SF002-96-1 at the indicated concentrations for 48 h. Cell viability was determined in triplicate using the XTT colorimetric assay. (B

Asymmetric synthesis of host-directed inhibitors of myxoviruses

• Terry W. Moore,
• Kasinath Sana,
• Dan Yan,
• Pahk Thepchatri,
• John M. Ndungu,
• Manohar T. Saindane,
• Mark A. Lockwood,
• Michael G. Natchus,
• Dennis C. Liotta,
• Richard K. Plemper,
• James P. Snyder and
• Aiming Sun

Beilstein J. Org. Chem. 2013, 9, 197–203, doi:10.3762/bjoc.9.23

• transcription unit in the genome of a measles virus (MeV) recombinant; and (3) a colorimetric assay that measures reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) as a surrogate for general cytotoxicity, reported here as CC50. Additionally, the aqueous solubilities of the

Molecular recognition of organic ammonium ions in solution using synthetic receptors

• Andreas Späth and
• Burkhard König

Beilstein J. Org. Chem. 2010, 6, No. 32, doi:10.3762/bjoc.6.32